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. 2021 May 25;218(7):e20210223. doi: 10.1084/jem.20210223

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© 2021 Ho et al.

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Figure 3. — Exposure to young blood in heterochronic parabiosis mice does not functionally rejuvenate old HSCs. (A) Experimental setup for the (3/24) parabiosis cohorts. Survival results after surgery are shown in Fig. S2 A. (B) Representative images (left) and quantification of MCM2- and DCX-positive cells (right) in the DG of O-Iso and O-Het parabionts. (C) HSC frequency in indicated mice. Age-matched non-parabiosed young (Y) and old (O) controls are included for comparison. (D) CD150 mean fluorescence intensity (MFI) levels for the indicated HSC populations. (E) Regenerative capacity for the indicated HSC populations following transplantation into lethally irradiated recipients (250 HSCs/recipient). Results show overall engraftment in the peripheral blood over time. (F) Lineage distribution for the indicated HSC populations at 4 mo after transplantation in blood (right) and BM (left). Data are means ± SD; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.