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. 2021 May 25;218(7):e20210223. doi: 10.1084/jem.20210223

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© 2021 Ho et al.

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Figure 6. — Migration to young BM niches does not functionally rejuvenate old HSCs. (A) Experimental setup for the analyses of old HSCs homed into Y-Het mice (O/Y HSCs) in the (3/24) cohorts and analyzed after 1 mo of parabiosis. (B) Frequency of HSC crossover in the indicated parabiosis cohorts. Of note, three Het pairs were initially injected with G-CSF. (C) Total numbers of HSCs showing both host and crossover O/Y HSCs. (D) Percentage of crossover in the HSC/MPP compartments of indicated mice. (E) HSC frequency in indicated mice. (F) CD150 mean fluorescence intensity (MFI) levels for the indicated HSC populations. (G) Regenerative capacity following transplantation in lethally irradiated recipients (250 HSCs/recipient) showing overall engraftment in the peripheral blood over time (left) and lineage distribution at 4 mo after transplantation in blood and BM (left) for the indicated HSC populations. Data are means ± SD; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. HSC crossover data in B and D are part of Fig. 2, B and F, and are shown for comparison.