Figure S5.

p38-induced phosphorylation of EGFR Ser1015 is dependent on LL1010/1011 and independent on AP2. (A) PAE cells expressing WT EGFR-GFP or the LL1010/11A mutant were incubated with vehicle (Ctrl), 10 ng/ml EGF (E), or 100 nM anisomycin (A) for 15 min at 37°C. Cells were lysed, and lysates were probed by Western blotting (WB) with antibodies to pS1046/47, pS1015, pY1068, activated p38 (p-p38), pERK1/2, and α-actinin (loading control). (B) HeLa/FAP-EGFR cells were transfected with nontargeting (NT) or μ2 siRNAs. 4 d later, the cells were stimulated with vehicle (Ctrl), 4 ng/ml EGF (E), 10 ng/ml TNFα (T), or 100 nM anisomycin (A). Cells were lysed, and lysates were immunoblotted with antibodies to pS1015, α-adaptin, and Grb2 (loading control).