Figure 1.

Experimental protocol. Upper panel—a schematic representation of the regional ischaemia induced by the LAD occlusion. Lower panel—protocols of heart perfusion and ventricular sample collection. 8-Br was perfused for 5 min at the concentration of 10 μM followed by a 5 min washout period. Hearts of C-Mito and 8-Br-Mito groups (n = 5 and 7, respectively) were not subjected to ischaemia and used for isolation of mitochondria. C-Pre and C-Post—control groups of hearts for the First and Second series of experiments (n = 7 and 6, respectively). 8-Br-Pre and 8-Br-Post—groups of hearts treated with 8-Br either before regional ischaemia (first series of experiments) or at the end of 30 min regional ischaemia (second series of experiments).