Figure 3.

Effects of PCs on the Nrf2/ARE pathway in H₂O₂-treated PC12 cells. (a) Protein levels of Nrf2 and Keap1, as determined by Western blotting; (b) Nrf2/GAPDH protein relative expression (ratio to control); (c) Keap1/GAPDH protein relative expression (ratio to control); (d) protein expression levels of nuclear Nrf2 and cytoplasmic Nrf2, as determined by Western blotting; (e) nuclear Nrf2/LaminB protein relative expression (ratio to control); (f) cytoplasmic Nrf2/GAPDH protein relative expression (ratio to control); (g) protein levels of HO-1, NQO1, glutamate-cysteine ligase catalytic subunit (GCLC), and GCLM, as determined by Western blotting; (h) HO-1/GAPDH protein relative expression (ratio to control); (i) NQO1/GAPDH protein relative expression (ratio to control); (j) GCLC/GAPDH protein relative expression (ratio to control); (k) GCLM/GAPDH protein relative expression (ratio to control). Data are expressed as the mean ± SD. All experiments were conducted three times. Values with different letters above are significantly different (p < 0.05, one-way ANOVA).