Figure 4.

Survival rate and heartbeat of zebrafish after being treated with SFPS or/and with LPS. (A) Survival rate of zebrafish, and (B) heartbeat of zebrafish. At 7–9 hpf, the zebrafish embryos were treated with different concentration of SFPS (25, 50, and 100 μg/mL) and stimulated with LPS (10 μg/mL) until 24 hpf. The heartbeat rate was measured at 2 dpf and the survival rate was measured at 3 dpf. The experiments were conducted in triplicate and data are expressed as mean ± SE. * p < 0.05, ** p < 0.01 as compared to LPS-treated group and ^## p < 0.01 as compared to control group.