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Activity of T7 RNAP mutants on the natural template. (a) Expression level of T7 RNAP mutants in the coupled2 cell-free system. Western blot of the WGE lysate where T7 RNAP mutants were synthesized. (b) Activity of the mutants of T7 RNAP normalized on the amount of T7 RNAP synthesized in the coupled2 cell-free system using natural template, n = 3, mean ± SD.
