Table 1.
Mutants of T7 RNAP, tested in the study.
| Mutant | Transcription Activity | References |
|---|---|---|
| P266L | Increased thermal stability and promoter clearance | [53] |
| N433Q | Increased thermal stability | [54,55] |
| G542V | Involved in interactions with the 2′-hydroxyl moiety of ribonucleotides | [50] |
| R627S | Increased thermal stability, interactions with phosphate groups of NTPs, incorporation of ddNTP | [54,55] |
| S633P | Increased thermal stability | [54,55,56] |
| H772A | [56] | |
| F849I | Increased thermal stability | [54,55,56] |
| F849A | Increased thermal stability | [54,55,56] |
| F849Y | Increased thermal stability | [54,55,56] |
| FAL: P266L/Y639F/H784A | Increased thermal stability, can accept 2′-O-methyl triphosphates | [31,54,57] |