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. 2021 May 14;22(10):5214. doi: 10.3390/ijms22105214

Figure 3.

Figure 3

Differential release of miRNAs by single cultures of brain microvascular endothelial cells (BMECs) or breast cancer cells (BCCs), and mixed BMEC–BCC cultures. Single cultures of 4T1 cells (BCCs), b.End5 cells (BMECs) and mixed cultures of b.End5 and 4T1 (previously labelled with CellTracker™ Green) cells, were performed under physiological shear stress for 1, 3, 6 and 24 h, after which the media were collected and processed for real time quantitative PCR of the indicated miRNAs. The results are presented as fold-change vs. 1 h of each culture system (with the exception of miR-181a-1-3p in b.End5 single cultures, which were normalized for 24 h), and highlight significant alterations with time and between cultures for miR-194-5p (A), miR-92a-1-5p (B), miR-205-5p (C) and miR-181a-1-3p (D). Statistical differences are denoted as * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 1 h of each culture, and by # p < 0.05, ## p < 0.01, ### p < 0.001 between indicated conditions, determined by one-way ANOVA within each culture against time, and by two-tailed unpaired Student’s t-test for each time point between cultures. Data represented are means ± SEM, n = 3.