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. 2021 May 14;22(10):5214. doi: 10.3390/ijms22105214

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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miR-194-5p is expressed by brain microvascular endothelial cells (BMECs), breast cancer cells (BCCs) and during BMEC–BCC interaction. Single cultures of 4T1 cells (BCCs), b.End5 cells (BMECs) and mixed cultures of b.End5 and 4T1 cells (previously labelled with CellTracker™ Green) were performed under physiological shear stress for 1, 3, 6 and 24 h, after which cells were fixed and processed for in situ hybridization (ISH) for miR-194-5p and nuclei (blue) were counterstained with Hoechst 33342. Insets highlight major cellular alterations of miRNA expression in both cell populations (BMECs: left; BCCs: right) over time. ISH for miR-194-5p revealed a bluish coloration in BMECs, BCCs and particularly in BCCs interacting with BMECs.