Figure 3.

Rhodamine B-loaded PLGA RG-503H nanoparticles internalise into human endothelial cells. (A) Human endothelial cells were incubated over 18 hrs (hours) with Rhodamine B-loaded PLGA RG-503H nanoparticles to assess their ability to internalise. Cells were labelled with a GAPDH 14C10 monoclonal antibody for cytoplasm staining, 4′,6-diamidino-2-phenylindole for nucleus staining and Rhodamine B fluorescent dye for nanoparticle staining. Nanoparticles were found to accumulate within the cytoplasm after 18 hrs. Cells were visualised using Zeiss Axio Observer Z1 immunofluorescence microscope. Representative images of each channel alongside a merged image are shown with scale bar = 10 µM. (B) Z-stack images showing localisation of nanoparticles within endothelial cells. Both angles confirm nanoparticles have internalised and do not aggregate or clump on the cell exterior.