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. 2021 May 17;14(5):478. doi: 10.3390/ph14050478

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of 5-fluorouracil (5FU) and 5FU with BGP-15 (5FU+BGP) treatment on skeletal muscle oxidative capacity and mitochondrial dynamics signalling. Succinate dehydrogenase (SDH) staining was performed on tibialis anterior (TA) cross-sections. (A) SDH representative images displayed, with data presented as (B) overall SDH intensity and (C) SDH intensity separated based on oxidative fibre phenotype, i.e., low oxidative, more oxidative and highly oxidative. TA muscle homogenate was analyzed for (D) citrate synthase (CS) activity, as a marker of mitochondrial density. Further, TA homogenate was utilized in Western blotting experiments with samples probed for (E) HSP-70, (F) PARP-1 and (G) a suite of proteins related to mitochondrial dynamics including; TFAM, PGC-1, PGC-1, OPA1 and DRP1. Protein expression was normalised to total protein derived from Coomassie Brilliant Blue (CBB) staining and presented relative to vehicle (VEH) control group. (H) Western blotting and CBB representative images are displayed. * = p < 0.05; n = 6–8 for SDH histology; n = 4 for VEH and n = 6–8 for 5FU and 5FU+BGP groups for CS activity and Western blotting. Data are mean ± SEM.