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. 2021 May 17;14(5):478. doi: 10.3390/ph14050478

Figure 6.

Figure 6

The effect of BGP-15 (BGP), 5-fluorouracil (5FU) and 5FU with BGP (5FU+BGP) treatment on HSP-70 expression and cell viabilityC2C12 myotubes were treated with BGP, 5FU and 5FU with BGP before being prepared as lysates for Western blotting experiments. (A) C2C12 lysates were then probed for HSP-70, with protein expression normalised to total protein derived from Coomassie Brilliant Blue (CBB) staining data and data presented as a percentage of the vehicle control group (VEH). (B) Cell viability was analyzed via the resazurin cell viability assay, with data presented as a percentage of VEH. (C) Representative images for the cell viability assay are displayed. Scale bar = 100 m. * = p < 0.05; n = 3–4 for all C2C12 experiments. Data are mean ± SEM.