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Kinetics of binding of 3.2 U/L amylase in surgically-placed drain effluent solution to the POF-biosensor. During the sample incubation (t = 8 min) the optical minimum shifts were recorded and plotted over time (inset). Eight minutes showed the endpoint of the kinetics and was chosen as the incubation time for all the measurements. The error bars were calculated on a same POF-biosensor platform and equal to 0.2 nm.
