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. 2021 May 14;13(5):724. doi: 10.3390/pharmaceutics13050724

Figure 6.

Figure 6

Evaluation of histologic features of defects in the rat femur bone, Von Kossa’s Staining under conventional light microscopy (A–D) and Fluorescent Labeling under Confocal Laser Scanning Microscopy (A’–D’). Groups: G1: Control—no implant; G2: Co—Co-28Cr-6Mo alloy; G3: Co PIII—Co-28Cr-6Mo alloy with surface nanostructured by PIII; G4: Co+Ti PIII—Co-28Cr-6Mo alloy with surface coated with Ti and nanostructured by PIII. All groups show bone neoformation (red star) from the defect margins being more intense in G4 since it involves most of the metallic implant and proportionally with a lower volume of connective tissue, essential for osseointegration. The Von Kossa technique demonstrated the mineralization of newly formed bone. Fluorescence was similar for all groups with new bone formation from the margins of the lesion, and there is a predominance of green over red. Metallic implant (MI). Bar: 100 µm.