Table 1.
Average relative abundance and prevalence of fungal and bacterial ASVs across 103 Kombucha SCOBY samples as determined by metabarcoding analysis, compared with results of shotgun-sequencing of the synthetic ‘meta’-SCOBY sample.
| Taxa (ASV) | Metabarcoding | Shotgun Sequencing | |
|---|---|---|---|
| Relative Abundance | Prevalence 1 | Proportion of Kmers 2 | |
| Fungi | |||
| g__Brettanomyces | 0.813 ± 0.259 | 0.990 | 0.8884 |
| g__Zygosaccharomyces | 0.068 ± 0.170 | 0.625 | 0.0173 |
| s__Starmerella davenportii | 0.047 ± 0.124 | 0.481 | 0.016 |
| s__Lachancea fermentati | 0.038 ± 0.141 | 0.385 | 0.0086 |
| k__Fungi | 0.012 ± 0.050 | 0.183 | NA |
| f__Saccharomycetales_unidentified | 0.008 ± 0.072 | 0.019 | NA |
| g__Candida | 0.007 ± 0.062 | 0.019 | 0.0005 |
| g__Hanseniaspora | 0.004 ± 0.021 | 0.125 | 0.0038 |
| g__Pichia | 0.001 ± 0.011 | 0.010 | 0.0043 |
| p__Ascomycota | 0.001 ± 0.005 | 0.058 | NA |
| g__Kregervanrija | 0.001 ± 0.004 | 0.019 | 0.0076 |
| Bacteria | |||
| g__Komagataeibacter | 0.809 ± 0.299 | 0.971 | 0.7088 |
| Lactobacillales total | 0.129 | 0.0589 | |
| f__Lactobacillaceae | 0.072 ± 0.167 | 0.394 | NA |
| o__Lactobacillales_unidentified | 0.050 ± 0.117 | 0.327 | NA |
| g__Oenococcus | 0.007 ± 0.061 | 0.077 | 0.019 |
| g__Tanticharoenia | 0.039 ± 0.130 | 0.462 | 0.0003 |
| g__Acetobacter | 0.012 ± 0.055 | 0.173 | 0.117 |
| f__Acetobacteraceae | 0.006 ± 0.030 | 0.144 | NA |
| f__Bacillaceae_unidentified | 0.005 ± 0.040 | 0.029 | 0.0112 |
1 Proportion of SCOBY where relative abundance of taxa exceeded 0.1%. 2 Proportion of kmers mapped to taxa (at metabarcoding ASV-defined levels) within relevant microbial kingdom. For ASV’s unassigned at a comparable level, kmer proportions are not reported (NA).