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. 2021 May 14;10(5):1200. doi: 10.3390/cells10051200

Figure 2.

Figure 2

S1P1 in HLMVECs from Non-Smokers and Smokers. (a) Relative S1P1 mRNA expression in HLMVECs isolated from non-smoker (n = 6) and smoker (n = 5) donors measured by RTqPCR, normalized to the housekeeping gene human 18S (H18S) using 2−∆∆CT method and expressed as a fold-change versus control (non-smoker). ** p = 0.0035; 2-tailed unpaired Student’s t-test. (b) Representative Western blot and densitometry analysis of S1P1 protein abundance relative to vinculin loading control in non-smoker and smoker HLMVECs (n = 4; 20 µg protein/well). * p = 0.01; 2-tailed unpaired Student’s t-test. All graphs show data points, each representing an individual donor; horizontal lines are mean ± SEM.