Table 7.

Primers and probe sequence orientation for recognition of SARS-CoV-2 virus RNA.

Name	Target Genes	Sequence 5′→3′	Nucleotide position	Target Genes	Sequence 5′→3′	Nucleotide Position
Forward Primer:	ORF 1ab	CCCTGTGGGTTTTACACTTAA	13342-13362	N gene	GGGGAACTTCTCCTGCTAGAAT	28881-28902
Reverse Primer:		ACGATTGTGCATCAGCTGA	13442-13460		CAG ACATTTTGCTCTCAAGCTG	28958-28979
Immobilized Mutant Probe (sh):		FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ	13377-13404		FAM-TTGCTGCTGCTTGACAGATT-TAMRA	28934-28953
Matching type (mr) Sequence:		TCCAACCTTTCCACATACCGCAGCGGA	-		AACTCTGTCAAGCAGCAGCAA	-
Mismatching type (wt) Sequence:		TCCAAGCAAACCCAATACCGCAGCGGA	-		AACTCTACTAATTAGCAGCAA	-