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The coding potential and subcellular distribution of CD45 and PEBP1P3 RNA. (A) The coding potential predicted with the online web server CPC. (B) Identification of the CD45 antisense RNA PEBP1P3 in HuT 78 cells through gene-specific primer (GSP) RT-PCR and sequencing. (C) RT-PCR and agarose gel electrophoresis of CD45 isoforms and PEBP1P3. (D) The subcellular distribution ratio of RNA determined by RT-qPCR after the separation of the nuclear and cytoplasmic fractions in HuT 78 cells.
