Figure 3.
CD137 stimulation reduces renal inflammation after C. albicans infection. (A–G) C57BL/6 mice were injected intraperitoneally with 200 μg of anti-CD137 or control rat IgG antibody 1 day before infection with 3 × 105 CFU of C. albicans. (A) Gross morphology. (B) Representative H&E staining of 3-d post-infection kidneys. Black arrowheads indicate representative nodules. Magnification: 40×. (C) Changes in kidney weight (n = 6 mice per group). In some experiments, mice were injected with 200 μg of control or anti-CD137 antibody without infection and kidney weight was measured 5 days after injection (n = 3 mice per group). (D–F) Levels of IL-6 and TNF-α (D), CCL2 (E), and CXCL1 and CXCL2 (F) in kidney lysates at 3-d post-infection (n = 5 mice per group). (G) Numbers of infiltrating inflammatory monocytes and neutrophils in 3-d post-infection kidneys (n = 5 per group). Results are representative of 3 experiments. * p < 0.05; ** p < 0.01 between the two groups at the indicated time points.