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. 2021 May 16;10(5):1215. doi: 10.3390/cells10051215

Figure 8.

Figure 8

The C-terminal menin protein colocalizes with SYT-1 at presynaptic terminals and PSD-95 at post-synaptic terminals in hippocampal neuronal cultures. (A) High magnification confocal image of a synaptic ROI at DIV 7(n = 18 images, 5 independent samples, representative image), neuronal cell cultures were labeled with α-C-terminal menin (Ai), PSD-95 (Aii; postsynaptic marker), (Aiii) shows merged channels. (B) α-C-terminal menin, (Bi) labeled with α-SYT-1 (Bii; presynaptic marker) (Biii) shows merged channels (n = 22 images, 12 independent samples, representative image). Arrowheads, colocalization of C-menin with PSD-95 and α-SYT-1 in (A) and (B) respectively. Scale bar, (A,B) 10 μm. (C) Summary data, Pearson’s co-efficient, temporal pattern showing degree of colocalization of Synaptotagmin (SYT-1; presynaptic), PSD-95 (postsynaptic) puncta with C-menin in DIV 4, DIV 6, DIV 7, DIV 10 and DIV 12. Red dotted line indicates the literature value of significant DOC. Asterisks, statistical significance (one-way ANOVA followed by post-hoc Tukey test); ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns ≥ 0.05 See also Tables S13a,b and S14a,b.