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. 2021 May 16;10(5):1215. doi: 10.3390/cells10051215

Figure 10.

Figure 10

Menin protein colocalizes with nicotinic cholinergic receptors (nAChR) specific subunits α5 and α7 in a spatiotemporal pattern in a whole mouse brain. (A) Confocal high magnification image of a synaptic ROI of an adult brain slice (n = 15 images, 6 independent samples, representative image), brains slices were labeled with nAChR specific subunit α5 (Ai), α-C-terminal menin (Aii), (Aiii) shows merged channels. (B) labeled with α7 labeling α-bungarotoxin (Bi), α-C-terminal menin α5 (Bii), (Biii) shows merged channels (n = 15 images, 6 independent samples, representative image). Arrowheads, colocalization of C-menin with α5 and α7 in (A) and (B) respectively. Scale bar, (A) 25 μm (B) 20 μm. (C,D) Summary data, Pearson’s co-efficient, temporal pattern degree of colocalization of α5 (nAChR specific subunit), α5 (nAChR specific subunit) respectively in puncta with C-menin in E12.5, E15.5, E18, P10 and adult mouse brain. Red dotted line indicates the literature value of significant DOC; (one-sample t-test). Asterisks, statistical significance (one-way ANOVA followed by post-hoc Tukey test); ns ≥ 0.05, * < 0.01, *** p < 0.001, **** p < 0.0001. See also Supplementary Table S19a–f.