Figure 7.

Renin-b protects H9c2 cells from accumulation of cytosolic reactive oxygen species (ROS) induced by OGD. (A) Representative histograms, (B) mean fluorescence intensities (FLI) and (C) mean percentages of dihydroethidium (DHE)-positive pIRES control, renin-b, and renin-a cells. Cells were exposed to basal (non-treated) conditions, glucose starvation alone, anoxia alone, and the combination of glucose starvation and anoxia (OGD) for 24 h. Then, cells were incubated with the DHE fluorophore to detect cytosolically localized ROS. Under OGD, the ROS content was increased as detected by an increased mean FLI in a subpopulation (DHEhigh-positive cells) of pIRES control cells. In renin-b and renin-a cells exposed to OGD the percentage of DHEhigh-positive cells was reduced compared to pIRES control cells. Data represent mean values ± SEM of 9 experiments. Differences were considered significant using two-way ANOVA with Bonferroni correction (p-values see text). (*): effects of the intervention (medium conditions) within the same cell line; (#): effects of renin-b or renin-a overexpression, respectively, compared with pIRES control cells on same intervention; (§): effects of renin-a overexpression compared with renin-b cells on same intervention.