Table 2.

Primer sequence and amplification conditions.

Gene	Primers Sequence	Conditions	ProductSize (bp)
Fusobacterium nucleatum	5′-AGAGTTTGATCCTGGCTCAG-3′ 5′-GTCATCGTGCACACAGAATTGCTG-3′	5″ at 95 °C, 16″ at 55 °C, 8″ at 72 °C for 40 cycles	407
Porphyromonas gingivalis	5′-TGTAGATGACTGATGGTGAAAACC-3′ 5′-ACGTCATCCCCACCTTCCTC-3′	5″ at 95 °C, 5” at 52 °C, 4″ at 72 °C for 40 cycles	198
Candida albicans	5′-TTTATCAACTTGTCACACCAGA-3′ 5′-GGTCAAAGTTTGAAGATATACGT-3′	10″ at 95 °C, 10″ at 58 °C, 15″ at 72 °C for 30 cycles	354