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. 2021 May 17;10(5):612. doi: 10.3390/pathogens10050612

Figure 3.

Figure 3

(a) PCR and RT-PCR experiments on E. variegatus dissected guts with EvaTO_phage1 primers. Lanes 1–3: RT-PCR on RNA extracted from three pools (five samples each) of E. variegatus guts. Lanes 6–8: PCR on the same RNA sample as in lanes 1–3 without the retrotranscription step. Lane 4: PCR on DNA extracted from a pool of five E. variegatus guts. Lane 5: negative control (no DNA) (b) TEM micrograph of siphovirus-like particles observed in negatively stained crude extract from dissected gut of E. variegatus Torino; bar = 100 nm.