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. 2021 May 17;10(5):612. doi: 10.3390/pathogens10050612

Table 1.

ViPTree Gene ORF Prediction and Blastx Analysis of the 12 Selected Phage Sequences.

Transcripts IDs and Predicted ORFs Length
(bp)
Range RPK Hit Description
[Organism]
% Identities E-Value % Query Coverage
MW965290 5544 111.7
ORF1 398 1–398 WP_195316289.1 phage tail tape measure protein [Serratia marcescens] 70 2 × 10−51 97
ORF2 # 357 398–754 WP_039351538.1 phage tail protein [Pectobacterium fontis] 90 1 × 10−75 97
ORF3 # 753 804–1556 MBG6243408.1 phage minor tail protein L [Candidatus Symbiopectobacterium sp. Dall1.0] 96 0.0 100
ORF4 # 576 1713–2288 MBG6243407.1 peptidase P60 [Candidatus Symbiopectobacterium sp. Dall1.0] 95 8 × 10−165 94
ORF5 # 606 2272–2877 WP_104212022.1 tail assembly protein [Pectobacterium brasiliense] 81 2 × 10−85 100
ORF6 2611 2934–5544 WP_104212026.1 phage tail protein [Pectobacterium brasiliense] 91 2 × 10−70 100
MW965281 2188 112
ORF1 318 255–572 WP_021179416 fimbrial protein TcfA [Serratia fonticola] 62 4 × 10−29 90
ORF2 # 252 557–808 WP_146751463.1 ANR family transcriptional regulator [Enterobacter cloacae complex] 56 5 × 10−7 60
ORF3 # 1089 859–1947 WP_187497555.1 phage tail protein [Pantoea Psp39-30] 43 2 × 10−82 98
MW965289 1777 128.3
ORF1 99 1–99 EFC4054519.1 HK97 family phage prohead protease [Escherichia coli] 81 2 × 10−9 100
ORF2 # 1227 109–1335 MBG6243159.1 phage major capsid protein [Candidatus Symbiopectobacterium sp. Dall1.0] 85 0.0 99
ORF3 # 300 1426–1725 WP_044208854.1Phage gp6-like head-tail connector family protein [Pectobacterium odoriferum] 87 3 × 10−57 100
MW965291 6115 213.4
ORF1 580 1–580 WP_108703399 Terminase small subunit [Enterobacter hormaechei] 96 2 × 10−86 96
ORF2 # 1659 583–2241 WP_108703400 terminase large subunit [Citrobacter europaeus] 98 0.0 100
ORF3 # 1935 2324–4258 WP_135684645.1 phage major capsid protein [Klebsiella pneumoniae] 95 0.0 99
ORF4 # 168 4297–4464 WP_181941880.1 hypothetical protein [Klebsiella pneumoniae] 89 8 × 10−28 100
ORF5 # 1359 4464–5822 NIC64170.1 phage portal protein [Klebsiella pneumoniae] 94 0.0 100
ORF6 297 5819–6115 RTO54147.1 phage gp6-like head-tail connector protein, partial [Enterobacter hormaechei] 80 8 × 10−47 100
MW965287 1419 71.2
ORF1 482 1–482 MBD2797976.1 HK97 family phage prohead protease [Xenorhabdus sp. 18] 72 6 × 10−74 96
ORF2 953 467–1419 QBY47020.1 phage portal protein [Arsenophonus nasoniae] 92 0.0 100
MW965282 770 61.0 WP_187497555.1 putative phage tail protein [Plautia stali symbiont] 99 1 × 10−158 84
MW965283 3268 114.1 WP_113869621.1 phage tail tape measure protein [Brenneria salicis] 82 0.0 82
MW965286 * 251 47.8 SPW64604.1 putative head-tail adaptor [Escherichia coli] 98 1 × 10−20 73
MW965288 768 50.8 MBJ9599707.1 phage portal protein [Citrobacter werkmanii] 99 4 × 10−179 94
MW965292 # 1852 123.1 WP_164114194.1 phage major capsid protein [Serratia marcescens] 63 0.0 77
MW965285 299 23.4 WP_010281992.1 portal protein [Pectobacterium brasiliense] 95 8 × 10−55 92
MW965284 * 345 46.4 SUH06759.1 portal protein [Salmonella enterica subsp. enterica] 87 1 × 10−27 66

RPK: read counts divided by the length of each transcript in kilobases. * putative pseudogene (presence of frameshifts). # Complete CDS. Grey shaded cells represent different transcript IDs.