Fig. 1.

The binding specificity of mAbs A1, B1, C1, and 9E-1 against ZIKV E protein. a The His-tagged ZIKV E protein (100 ng) was coated on a 96-well plate and then subjected to ELISA analysis with mAbs Anti-His, A1, B1, C1, or 9E-1, respectively. The detailed experimental procedures were described in the “Materials and methods” section. The results of the ELISA analysis were evaluated quantitatively by measuring the absorbance at 450 nm. Values are means ± S.D. from three independent experiments. b The recombinant ZIKV E protein expressed in Sf21 insect cells (2 μg) was separated on SDS-PAGE and then transferred to a PVDF membrane for performing the WB analysis with mAbs Anti-His, A1, B1, C1, or 9E-1, respectively. The numbers labeled on the left of the designated ticks are the molecular weight standards in kDa unit