Fig. 2.
Determination of the binding affinity of mAbs A1, B1, C1, and 9E-1 against ZIKV E protein. The kinetics assays by surface plasmon resonance (SPR) analysis were performed with a series of two-fold diluted mAbs A1, B1, C1, and 9E-1 solutions (starting from 1,000 to 3.90625 nM) and the purified recombinant ZIKV E proteins, which were covalently immobilized on the surface of the Sensor Chip CM5 as described in the “Materials and methods” section. a The SPR sensogram of mAb A1 interacting with ZIKV E protein. b The SPR sensogram of mAb B1 interacting with ZIKV E protein. c The SPR sensogram of mAb C1 interacting with ZIKV E protein. d The SPR sensogram of mAb 9E-1 interacting with ZIKV E protein. e The list of kon, koff, and KD values of mAbs A1, B1, C1, and 9E-1 against ZIKV E protein