Fig. 5.

Alignment of the binding epitopes of mAbs A1 (a), B1 (b), C1 (c), and 9E-1 (d) with other flavivirus E proteins. DENV, dengue virus; WNV, West Nile virus; YFV, yellow fever virus; JEV, Japanese encephalitis virus. The NCBI accession numbers of the E proteins derived for the indicated virus strains are provided in the parentheses. e The recombinant E proteins of ZIKV (2 μg), DENV2 (1.75 μg), DENV3 (0.42 μg), and DENV4 (0.78 μg) were separated on SDS-PAGE and then transferred to a PVDF membrane for performing the WB analysis with mAbs Anti-His, A1, B1, C1, or 9E-1, respectively. The numbers labeled on the left of the designated ticks are the molecular weight standards in kDa unit