Fig. 6.

Analysis of the binding affinity and neutralization activity of mAbs A1, B1, C1, and 9E-1 against ZIKV. a The virus virions of ZIKV isolate Thailand/1610acTw were coated on a 96-well plate and then subjected to ELISA analysis with mAbs, A1, B1, C1, or 9E-1, respectively. The results were examined quantitatively by measuring the absorbance at 450 nm. Values are means ± S.D. from three independent experiments. For conducting the PRNT experiments, PBS (b), a batch of two-fold serially diluted ZV-54 antibody solutions (starting from 10 μg/mL as indicated in the schematic diagram on the left) (c), and a batch of A1, B1, C1, 9E-1, or 1C6B (an Anti-H7N9 HA mAb) antibody solutions (starting from 100 μg/mL as indicated in the schematic diagram on the left) (d) were pre-incubated with 100 PFU of ZIKV for performing the PRNT assays as described in the “Materials and methods” section. The results showed that mAbs A1, B1, C1, and 9E-1 do not have ZIKV neutralizing activity