Fig. 7.

Detection of ZIKV E proteins and virions by LFIA. The LFIA strip (#1–#7) was composed of four main materials including sample pad, conjugate pad, nitrocellulose membrane, and absorbent pad. mAb B1 was conjugated with colloidal gold particles and loaded on the conjugate pad. mAb 9E-1 was loaded on the test line. Goat anti-mouse IgG antibody was loaded on the control line. The assay was conducted by adding PBS, the purified ZIKV E proteins (7-33 μg/mL), or ZIKV (6.3×10⁶ pfu/mL) on the sample pad to start the LFIA. The results were interpreted within 10 min. The colloidal gold particles can only be observed on the test line while using the purified ZIKV E proteins or ZIKV as the test sample