Figure 5.

Overexpression of E2F1 reverses the inhibitory effect of MCTS1 knockdown on lung adenocarcinoma cellular malignant behaviors. (A) Western blot analysis was performed to detect the protein expression levels of E2F1 and MCTS1. (B) The MTT assay was performed to assess the proliferation of H1573 and H1299 cells following transfection with si-NC, siMCTS1, siMCTS1+ pcDNA3.1 and siMCTS1+E2F1. (C) Cell migration and (D) apoptosis were investigated following E2F1 knockdown. *P<0.05, **P<0.01 vs. si-NC, si-NC+E2F1, si-NC+pcDNA3.1 or siMCTS1+pcDNA3.1. MCTS1, multiple copies in T-cell lymphoma-1; si, small interfering; NC, negative control.