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. 2021 May 17;11(5):1430. doi: 10.3390/ani11051430

Figure 7.

Figure 7

Validation of differentially expressed circRNAs in young (Piglet-D30) and sexual mature (Adult-D210) boar testes. Relative expression levels of circRNAs before and after RNase R treatment (A). Changes of the relative expression levels of linear RNA corresponding to circRNAs before and after RNase R treatment (B). Relative expression changes of circRNAs in testes before and after sexual maturity (C). Changes of the relative expression levels of linear RNA corresponding to circRNAs in the testes before and after sexual maturity. R− and R+ represent RNA treat with or without RNase R. The data were normalized against endogenous housekeeping gene EF1α1 and the Value for the blank control was set as one (D).