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. 2021 May 14;22(10):5213. doi: 10.3390/ijms22105213

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot analysis for Wnt/β-catenin/glycogen synthase kinase-3β (GSK3) proteins. (A): Western blot gels of the target genes in (1) saline group, (2) 1,2-dimethylhydrazine (DMH) control group, (3&4) DMH + nitazoxanide 100 and 200 mg/kg groups, (B): Column charts for Wnt, β-catenin and GSK3B proteins relative to β-actin. Data are mean ± standard deviation (SD) and analyzed by one-way analysis of variance (ANOVA) and Bonferroni’s test. ^δ Compared to saline group, ^Φ Compared to DMH control group, $Compared to DMH + nitazoxanide 100 group, p < 0.05.

Western blot analysis for Wnt/β-catenin/glycogen synthase kinase-3β (GSK3) proteins. (A): Western blot gels of the target genes in (1) saline group, (2) 1,2-dimethylhydrazine (DMH) control group, (3&4) DMH + nitazoxanide 100 and 200 mg/kg groups, (B): Column charts for Wnt, β-catenin and GSK3B proteins relative to β-actin. Data are mean ± standard deviation (SD) and analyzed by one-way analysis of variance (ANOVA) and Bonferroni’s test. ^δ Compared to saline group, ^Φ Compared to DMH control group, $Compared to DMH + nitazoxanide 100 group, p < 0.05.