Table 3.
Primer | Sequence | Annealing Temperature |
---|---|---|
P53 | FOR: 5′-CTTTGAGGTGCGTGTTTGTG-3′ REV: 5′-GTGGTTTCTTCTTTGGCTGG-3′ |
57 °C |
BCL-2 | FOR: 5′-GAGGATTGTGGCCTTCTTTG-3′ REV: 5′-ACAGTTCCACAAAGGCATCC0-3′ |
56 °C |
BAX | FOR: 5′-TTTGCTTCAGGGTTTCATCC-3′ REV: 5′-CAGTTGAAGTTGCCGTCAGA-3′ |
55 °C |
Casp-3 | FOR: 5′- TGGCCCTGAAATACGAAGTC-3′ REV: 5′- GGCAGTAGTCGACTCTGAAG -3′ |
56 °C |
Casp-8 | FOR: 5′- AATGTTGGAGGAAAGCAAT -3′ REV: 5′- CATAGTCGTTGATTATCTTCAGC -3′ |
51 °C |
Casp-9 | FOR: 5′- CGAACTAACAGGCAAGCAGC -3′ REV: 5′- ACCTCACCAAATCCTCCAGAAC -3′ |
58 °C |
β-actin | FOR: 5′-GCACTCTTCCAGCCTTCCTTCC-3′ REV: 5′-GAGCCGCCGATCCACACG-3′ |
60 °C |