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. 2021 May 14;22(10):5213. doi: 10.3390/ijms22105213

Table 3.

The set of polymerase chain reaction (PCR) primers for the selected genes.

Primer Sequence Annealing Temperature
P53 FOR: 5′-CTTTGAGGTGCGTGTTTGTG-3′
REV: 5′-GTGGTTTCTTCTTTGGCTGG-3′
57 °C
BCL-2 FOR: 5′-GAGGATTGTGGCCTTCTTTG-3′
REV: 5′-ACAGTTCCACAAAGGCATCC0-3′
56 °C
BAX FOR: 5′-TTTGCTTCAGGGTTTCATCC-3′
REV: 5′-CAGTTGAAGTTGCCGTCAGA-3′
55 °C
Casp-3 FOR: 5′- TGGCCCTGAAATACGAAGTC-3′
REV: 5′- GGCAGTAGTCGACTCTGAAG -3′
56 °C
Casp-8 FOR: 5′- AATGTTGGAGGAAAGCAAT -3′
REV: 5′- CATAGTCGTTGATTATCTTCAGC -3′
51 °C
Casp-9 FOR: 5′- CGAACTAACAGGCAAGCAGC -3′
REV: 5′- ACCTCACCAAATCCTCCAGAAC -3′
58 °C
β-actin FOR: 5′-GCACTCTTCCAGCCTTCCTTCC-3′
REV: 5′-GAGCCGCCGATCCACACG-3′
60 °C