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. 2021 May 14;9(5):552. doi: 10.3390/biomedicines9050552

Figure 2.

Figure 2

RT-PCR analysis of SMN transcripts across exons 4–8 in normal human fibroblasts transfected with 2′-O-methyl PS AOs (100, 50 and 25 nM). The full length transcript (FL, 404 bp) and exon skipped products (delta-7(Δ7), 350 bp and delta-5(Δ5), 308 bp) are indicated by the lines to the left of the gel, and the percentage of (a) Δ7-SMN or (b) Δ7-SMN and Δ5-SMN as determined by densitometry is shown below each lane for comparison to controls and untreated (UT) fibroblasts. A 100 bp size marker was used for size comparison and a no template RT-PCR control (NTC) was loaded in the final lane.