Table 2.
PCR conditions for human SMN and mouse Smn transcript amplification.
| Transcript Sizes | Primer | Primer Sequence | Temperature Profile |
|---|---|---|---|
|
SMN FL 404 bp Δ5 308 bp Δ7 350 bp |
Exon 4 Fwd Exon 8 Rev |
AGGTCTCCTGGAAATAAATCAG TGGTGTCATTTAGTGCTGCTCT |
55 °C 30 min 94 °C 2 min 25 cycles: 94 °C 40 s 56 °C 1 min 68 °C 1 min |
|
Smn FL 430 bp Δ5 334 bp Δ7 380 bp |
Exon 4 Fwd Exon 8 Rev |
GAAAGTCAAGTTTCCACAGACG CACCCCATCTCCTGAGACAGAGC |
55 °C 30 min 94 °C 2 min 28 cycles: 94 °C 40 s 60 °C 1 min 68 °C 1 min |