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Schematics of a rotary cell culture system (RCCS): (a) four-station rotator base with one 50 mL vessel and two 10 mL vessels; (b) at low rotation speeds, the cells will settle along the bottom of the vessel; (c) at very high rotational speeds, the cells will be subjected to a predominant centrifugal force, driving them towards the outer wall of the vessel; and (d) at a certain velocity range, the cells will be in suspension, in a simulated microgravity environment.
