Table 1.
Solvents | Dye | Photophysical Properties | ||||
---|---|---|---|---|---|---|
λabs a (nm) |
λem b (nm) |
∆λ c (nm) |
Φf d (n = 3) |
ε e (M−1 cm−1) |
||
DMSO | 3a | 417 | 532 | 115 | 0.69 ± 0.04 | 4.6 × 104 |
3b | 431 | 560 | 129 | 0.34 ± 0.04 | 3.1 × 104 | |
3c | 413 | 520 | 107 | 0.61 ± 0.04 | 1.0 × 104 | |
3d | 422 | 516 | 94 | 0.10 ± 0.02 | 4.6 × 104 | |
3e | 420 | 539 | 119 | 0.50 ± 0.02 | 3.2 × 104 | |
3f | 412 | 512 | 100 | 0.21 ± 0.03 | 3.5 × 104 | |
MeOH | 3a | 417 | 549 | 132 | 0.013 ± 0.006 | 3.0 × 104 |
3b | 428 | 567 | 139 | 0.012 ± 0.007 | 2.9 × 104 | |
3c | 414 | 546 | 132 | 0.017 ± 0.011 | 1.0 × 104 | |
3d | 423 | 555 | 132 | 0.024 ± 0.015 | 3.7 × 104 | |
3e | 417 | 547 | 130 | 0.012 ± 0.007 | 3.2 × 104 | |
3f | 414 | 549 | 135 | 0.027 ± 0.011 | 3.1 × 104 | |
PBS (3%w/w tween 80) |
3a | 416 | 529 | 113 | 0.15 ± 0.02 | 3.3 × 104 |
3b | 427 | 520 | 93 | 0.11 ± 0.02 | 3.7 × 104 | |
3c | 411 | 526 | 115 | 0.11 ± 0.02 | 1.3 × 104 | |
3d | 422 | 542 | 120 | 0.17 ± 0.03 | 4.4 × 104 | |
3e | 420 | 521 | 101 | 0.18 ± 0.03 | 2.8 × 104 | |
3f | 412 | 524 | 112 | 0.18 ± 0.02 | 3.4 × 104 |
a λabs = absorption maximum wavelength, b λem = emission maximum wavelength (excited at the maximum absorption wavelength), c ∆λ = stokes shifts (λem − λabs), d Φf = fluorescence quantum yields calculated by using fluorescein in 0.1 M NaOH as a standard (Φf = 0.95). e ε = molar absorptivity.