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. 2021 May 17;26(10):2979. doi: 10.3390/molecules26102979

Table 1.

Photophysical properties of chalcone 3af (2 µM) in different solvents.

Solvents Dye Photophysical Properties
λabs a
(nm)
λem b
(nm)
∆λ c
(nm)
Φf d
(n = 3)
ε e
(M−1 cm−1)
DMSO 3a 417 532 115 0.69 ± 0.04 4.6 × 104
3b 431 560 129 0.34 ± 0.04 3.1 × 104
3c 413 520 107 0.61 ± 0.04 1.0 × 104
3d 422 516 94 0.10 ± 0.02 4.6 × 104
3e 420 539 119 0.50 ± 0.02 3.2 × 104
3f 412 512 100 0.21 ± 0.03 3.5 × 104
MeOH 3a 417 549 132 0.013 ± 0.006 3.0 × 104
3b 428 567 139 0.012 ± 0.007 2.9 × 104
3c 414 546 132 0.017 ± 0.011 1.0 × 104
3d 423 555 132 0.024 ± 0.015 3.7 × 104
3e 417 547 130 0.012 ± 0.007 3.2 × 104
3f 414 549 135 0.027 ± 0.011 3.1 × 104
PBS
(3%w/w tween 80)
3a 416 529 113 0.15 ± 0.02 3.3 × 104
3b 427 520 93 0.11 ± 0.02 3.7 × 104
3c 411 526 115 0.11 ± 0.02 1.3 × 104
3d 422 542 120 0.17 ± 0.03 4.4 × 104
3e 420 521 101 0.18 ± 0.03 2.8 × 104
3f 412 524 112 0.18 ± 0.02 3.4 × 104

a λabs = absorption maximum wavelength, b λem = emission maximum wavelength (excited at the maximum absorption wavelength), c ∆λ = stokes shifts (λem − λabs), d Φf = fluorescence quantum yields calculated by using fluorescein in 0.1 M NaOH as a standard (Φf = 0.95). e ε = molar absorptivity.