Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 17;22(10):5253. doi: 10.3390/ijms22105253

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Formation of nitrosylated PE during the inhibition of ferroptosis by NO^● (a) Bar graph showing the decrease in cell death upon addition of ferroptosis inhibitor Ferrostatin-1 and DTPA NONOate in RAW 264.7 M2 macrophages treated with RSL3. Data are mean ± SD, n = 3 and no statistical significance was observed. (b) Mass spectrum showing the presence of two nitrosylated PE species, 1-OA-2-ETE(-NO)-PE (top panel) and 1-SA-2-ETE(-NO)-PE (bottom panel) in RAW 264.7 M2 macrophages treated with RSL3+Fer-1 (bottom section) and RSL3+DTPA NONOate (top section). Inset shows the magnified spectrum. (c) Quantities of 1-OA-2-ETE(-NO)-PE and 1-SA-2-ETE(-NO)-PE in RAW 264.7 M2 macrophages treated with RSL3+Fer-1 and RSL3+DTPA NONOate. Data are mean ± SD, n = 3 for RSL3+Fer-1 cells and 6 for RSL3+DTPA NONOate cells. p values are calculated using two-way ANOVA followed by Sidak post-hoc test.