Figure 1.

Graphical representation of approaches for NP discovery from environmental samples. Enrichment and evaluation of environmental samples are necessary steps prior to metagenomics analysis. Function-based metagenomics analysis includes library construction, heterologous expression, and function-based screening. Sequencing-based metagenomics analysis requires eDNA sequencing, DNA synthesis and assembly, and heterologous expression of target clones. Single-cell metagenomics analysis includes cell sorting, single cell genomic DNA amplification, genome sequencing, and DNA synthesis and assembly of NP biosynthetic gene clusters (BGGs) of interest, then expression and chemical identification.