. 2021 Mar;59(1):112–121. doi: 10.17113/ftb.59.01.21.6902

Table 2. Cycle threshold (Ct) values obtained for Tribolium castaneum in 5 g of wheat flour used for regression analysis.

Sample	m(DNA)/ng	N(insect equivalent)	Ct value
T. castaneum adult DNA diluted with wheat DNA	200	10	(16.8±0.02)^a
	100	5	(20.0±0.1)^a
	50	2.5	(21.67±0.09)^a
	25	1	(22.7±0.2)^a
	12.5	0.1	(27.0±0.2)^a
	1.25	0.01	(27.9±0.3)^a
	0.125	0.001	(28.84±0.08)^a
Wheat DNA	108	NA	34.4±1.2

^aSignificant difference between all Tribolium DNA mass and wheat DNA at p<0.01 level of significance. Each value is represented as mean±S.D. (N=3). NA=not available