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. Author manuscript; available in PMC: 2021 May 27.
Published in final edited form as: J Hepatol. 2020 Sep 17;74(2):394–406. doi: 10.1016/j.jhep.2020.09.010

Fig. 4. Biological impact of SAMe on survival and proliferation of normal and cystic cholangiocytes in culture.

Fig. 4.

Cell (A) viability (n = 6), (B) apoptosis (n = 4) and (C) proliferation (n = 3) in untreated or SAMe-treated NHCs and PHCs. (D) mRNA levels of epithelial and polarity markers in the presence/absence of SAMe. (E) Immunoblot and quantification of acetylated oi-tubulin in cultured PHCs, in the presence/absence of SAMe (n = 6). (F) Immunofluorescence images of acetylated α-tubulin in PHCs (primary dlium in green and nucleus in blue) in the presence/absence of SAMe (n = 24) (scale bars: 20 μm, 4 μm [cropped]). *p <0.05; **p <0.01; ***p <0.001 (one-way ANOVA Kruskal-Wallis, Mann-Whitney or 2-tailed t-tests). NHCs, normal human cholangiocytes; PHCs, polycystic human cholangiocytes; SAMe, S-adenosylmethionine.