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. 2021 May 17;11(5):742. doi: 10.3390/biom11050742

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of proline on ROS level and GSH concentration in pTr2 cells. (A) DCFH-DA positive populations were determined by flow cytometry analysis in a time course. (B) mRNA levels for GCLC and GSS. (C) Intracellular T-GSH and GSH. Data are expressed as the percentage of control group. Values are means ± SEMs, n = 3. Means at a time point without a common letter differ, p < 0.05. DCFH-DA, 2′,7′-Dichlorofluorescein diacetate; pTr2, porcine trophectoderm cells 2; GCLC, catalytic subunit of glutamate–cysteine ligase; GCLM, glutamate–cysteine ligase modifier subunit; GSH, glutathione; GSS, glutathione synthetase.