Table 1.
ARG markers used in the assay with sequence information of primers (F, forward primer; and R, reverse primer) and probes (P, TaqMan probe).
| ARG Markers | Resist to Antibiotics/Class | Sequences of Primes and Probes (5′–3′) | Ref. |
|---|---|---|---|
| mecA | Methicillin, Penicillin/β-lactam | F: CATTGATCGCAACGTTCAATTTAAT | [16] |
| R: TGGTCTTTCTGCATTCCTGGA | |||
| P: FAM-CTATGATCCCAATCTAACTTCCACATACC-MGBNFQ | |||
| ermB | Erythromycin/Macrolides | F: GGATTCTACAAGCGTACCTTGGA | [16] |
| R: GCTGGCAGCTTAAGCAATTGCT | |||
| P: FAM-CACTAGGGTTGCTCTTGCACACTCAAGTC-MGBNFQ | |||
| qnrS | Fluoroquinolones | F: CGACGTGCTAACTTGCGTGA | [17] |
| R: GGCATTGTTGGAAACTTGCA | |||
| P: FAM-AGTTCATTGAACAGGGTGA-MGBNFQ | |||
| sul1 | Sulfonamide | F: CCGTTGGCCTTCCTGTAAAG | [18] |
| R: TTGCCGATCGCGTGAAGT | |||
| P: FAM-CAGCGAGCCTTGCGGCGG-MGBNFQ | |||
| tetO | Tetracycline | F: AAGAAAACAGGAGATTCCAAAACG | [16] |
| R: CGAGTCCCCAGATTGTTTTTAGC | |||
| P: FAM-ACGTTATTTCCCGTTTATCACGGAAGCG-MGBNFQ | |||
| vanA | Vancomycin | F: CTGTGAGGTCGGTTGTGCG | [19] |
| R: TTTGGTCCACCTCGCCA | |||
| P: FAM-CAACTAACGCGGCACTGTTTCCCAAT-MGBNFQ | |||
| blaCTXM | Cefotaxime, Ceftazidime/β-lactam | F: ACCAACGATATCGCGGTGAT | [17] |
| R: ACATCGCGACGGCTTTCT | |||
| P: FAM-TCGTGCGCCGCTG-MGBNFQ | |||
| intI1 | Multidrug resistance | F: GCCTTGATGTTACCCGAGAG | [20] |
| R: GATCGGTCGAATGCGTGT | |||
| P: FAM-ATTCCTGGCCGTGGTTCTGGGTTTT-MGBNFQ |