Fig. 3. Confocal images of HeLa cells stained with 200 nM LysoTracker Red (LTR) for 10 min and then co-stained with 2 μM CSMPP for 10 min. Fluorescence images of (a) CSMPP; (b) LTR; (c) merged a and b; (d) merged brightfield with a and b; (e) the signal distribution diagram of the green channel for CSMPP and the red channel for LTR; (f) the fluorescence intensity profile extracted from the white arrow line in image c. Conditions: for CSMPP, λex = 405 nm, λem = 450–580 nm. For LTR, λex = 560 nm, λem = 650–700 nm. Scale bar is 10 μm.
