Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 20;22(1):542. doi: 10.3892/ol.2021.12803

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 1. — Preparation of TIM3-hFc and Gal9-rFc. (A) SDS-PAGE analysis of the purified TIM3-hFc, Gal9-rFc, mesothelin-hFc and mesothelin-rFc; 5 µg protein was loaded per lane. (B) ELISA analysis of biotinylated Gal9-rFc binding to immobilized TIM3-hFc. Biotinylated mesothelin-rFc was used as the isotype control. (C) ELISA analysis of biotinylated TIM3-hFc binding to immobilized Gal9-rFc. Biotinylated mesothelin-hFc was used as the isotype control. TIM3, T cell immunoglobulin and mucin domain 3; ELISA, enzyme-linked immunosorbent assay; Gal9, galectin-9.