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. Author manuscript; available in PMC: 2021 May 27.
Published in final edited form as: Cell. 2020 May 28;181(5):1188–1188.e1. doi: 10.1016/j.cell.2020.04.039
Ferroptosis-Related Assays and Tools
Assays and Tools Reagent method
BODIPY 581/591 C11 sensor for lipid ROS levels
LiperFluo fluorescent lipid peroxide reagent
TBARS assay detects MDA products of lipid per.
FENIX (fluorescence-enabled inhibited autoxidation) microplate-based assay to detect phospholipid peroxidation
metabolomics / lipidomics detects metabolites and lipids
GSH/GSSG ratio detection kit quantifies glutathione levels
Fe2+/Fe3+ quantification kit measures ferrous and ferric iron
anti-TfR1(3B8 2A1 & 3F3-FMA) detect TfR1, a ferroptosis marker
anti-MDA adduct (1F83) detects malondialdehyde
anti-4-HNE (ab46545) detects 4-hydroxynonenal