Skip to main content
. 2021 May 18;22(10):5293. doi: 10.3390/ijms22105293

Figure 1.

Figure 1

Lentiviral transduction efficiency in a human and a murine cell line compared to transfection efficiency. The cells were seeded at 100,000 cells per well in a 6-well plate and transduced 24 h later using different lentiviral vector suspension dilutions as shown in the graph. Murine C5N keratinocytes were harvested at 72 h after transduction, human MRC5Vi fibroblasts at 48 h after transduction for flow cytometry. Those would be the selected time points for puromycin selection after lentiCRISPR v2 application. To be able to compare transduction and transfection the same EGFP plasmid was used (pLJM1-EGFP). The transfected cells were harvested for flow cytometry measurements after 24 h. (A,B) show the results of C5N keratinocytes’ and MRC5Vi fibroblasts’ transduction and transfection efficiency, respectively. (C) compares the data of (A,B). Statistical analyses were performed using unpaired two-tailed Student’s t-test (n (transfected and transduced cells) = 4, n (control cells) = 7; * p < 0.1, ** p ≤ 0.01, *** p ≤ 0.001).