Figure 2.

Transduction efficiency of various established cell lines using lentiviral transduction of an EGFP-coding plasmid. Viruses were produced in HEK293T cells using psPAX2, pCMV-VSVg, and pLJM1-EGFP with Attractene transfection reagent. The different cell lines were seeded at 100,000 cells/well in a 6-well plate and transduced 24 h later using the filtered virus stock. Using fluorescence microscopy, pictures were taken at the indicated time points (EGFP: 1800 ms, scale bar in the lower right: 400 µm).